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KMID : 0379119890170040177
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Korean Journal of Mycology
1989 Volume.17 No. 4 p.177 ~ p.183
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Studies on the Light - Induced Mitochondrial ATP Synthase in Pleurotus ostreatus
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Abstract
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Mitochondria in Pleurotus ostreatus was purified by stepped sucrose density gradient centrifugation. The mitochondrial ATP synthase was investigated by various wavelength of the illumination at dark room for 30 min. The mitochondrial ATP synthase activity was stimulated 2.3 fold by 480 ㎚ illumination compared with the broad wavelength group. The mitochondrial ATP synthase activity according to various times of illumination was stimulated 4.2 fold for 15 min at 480 nm compared with the broad wavelength group. The optimum pH and optimum temperature of the mitochondrial ATP synthase were 7.5 and 56℃, respectively. The activity of this enzyme was stimulated by 0.5 mmol Fe^(2+), 1.0 mmol Fe^(3+) and 5.0 mmol k^+ ion, but inhibited by 0.1 mmol Na+ ion.
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